mini PCR: effects of PCR rxn volume and gDNA input validation

Visibility: Public
Publication: High-Throughput Miniaturized 16S rRUnpublished Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity Link
Journal: mSystems
Source: Link to the source page
Sample Number: 480

Samples in this project
  

SampleID Type Habitat Year Sequence type MNS1 MAS2
S_11432.echo5.0ul.500nl.zymo.2 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.500nl.zymo.3 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.500nl.zymo.4 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.ntc.3 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sediment.2 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sediment.3 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sediment.4 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sw.1 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sw.2 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sw.3 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.sw.4 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.zymo.2 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.zymo.3 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.50nl.zymo.4 Other_environment NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.sediment.1 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.sediment.3 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.sw.1 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.sw.2 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.sw.3 Marine NA 2017 16S V4 - -
S_11432.echo5.0ul.5nl.zymo.3 Other_environment NA 2017 16S V4 - -

1MNS (Microbiome Novelty Score): < 0.12: Non-novel; 0.12-0.2: Novel; > 0.2: Very novel; -: NA for samples before 2010

2MAS (Microbiome Attention Score): < 10: Low-attention; ≥ 10: High-attention; -: NA for samples before 2010